The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 16, 2007
Filed:
Jun. 06, 2005
Sergey Anatolievuch Avilov, Vladivostok, 690014, RU;
Vladimir Ivanovich Kalinin, Vladivostok, 690048, RU;
Alexandra Sergeevna Silchenko, Vladivostok, 690088, RU;
Dmitry Lvovich Aminin, Vladivostok, 690016, RU;
Irina Grigorevna Agafonova, Vladivostok, 690068, RU;
Valentin Aronovich Stonik, Vladivostok, 690048, RU;
Peter D. Collin, Sunset, ME (US);
Carl Woodward, Stonington, ME (US);
Sergey Anatolievuch Avilov, Vladivostok, 690014, RU;
Vladimir Ivanovich Kalinin, Vladivostok, 690048, RU;
Alexandra Sergeevna Silchenko, Vladivostok, 690088, RU;
Dmitry Lvovich Aminin, Vladivostok, 690016, RU;
Irina Grigorevna Agafonova, Vladivostok, 690068, RU;
Valentin Aronovich Stonik, Vladivostok, 690048, RU;
Peter D. Collin, Sunset, ME (US);
Carl Woodward, Stonington, ME (US);
Other;
Abstract
This present invention discloses use of a chloroform/solvent mixture extraction at different solvent-to-feed ratios, followed by evaporation and extraction with ethyl acetate/water; followed by chromatography of the water phase in Teflon or other non-polar resin and Silica gel column chromatography for recovering of individual triterpene glycosides (saponins) of high purity from the freeze dried or spray dried cooking water or dried powderized tissues of the industrial processed sea cucumber. A resulting glycoside Frondoside A stimulates lysosomal activity of peritoneal macrophages, phagocytosis and oxidative burst in the macrophages at concentrations significantly less than for acute toxicity, hemolysis and sea urchin embryo toxicity.