The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 26, 2006
Filed:
Feb. 17, 2000
Sunao Hisada, Shizuoka, JP;
Yukiko Ito, Shizuoka, JP;
Hiroyuki Matsumoto, Shizuoka, JP;
Kiyohito Shimura, Kanagawa, JP;
Kenichi Kasai, Kanagawa, JP;
Sunao Hisada, Shizuoka, JP;
Yukiko Ito, Shizuoka, JP;
Hiroyuki Matsumoto, Shizuoka, JP;
Kiyohito Shimura, Kanagawa, JP;
Kenichi Kasai, Kanagawa, JP;
Hamamatsu Photonics K.K., Hamamatsu, JP;
Abstract
A method for quantitatively detecting an antigen which comprises (1) a first step of providing an Fab' antibody having a uniform isoelectric point, said antibody forming an immune complex with an antigen in an analytical sample and being modified by adding an amino acid sequence comprising a charged amino acid residue and by being labeled with a fluorescent dye, (2) a second step of mixing the Fab′ antibody having a uniform isoelectric point with the analytical sample containing the antigen to obtain a mixture comprising the immune complex, (3) a third step of separating the mixture by performing electrophoresis in a carrier, (4) a fourth step of irradiating an excitation light which excites the fluorescent dye to the mixture separated in the third step to cause fluorescence in the immune complex, and (5) a fifth step of detecting the fluorescence.