The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2006
Filed:
Aug. 28, 1997
Stephen O'gorman, San Diego, CA (US);
Geoffrey Wahl, San Diego, CA (US);
Stephen O'Gorman, San Diego, CA (US);
Geoffrey Wahl, San Diego, CA (US);
The Salk Institute for Biological Studies, La Jolla, CA (US);
Abstract
Site-specific recombinases provide a means of efficiently manipulating chromosomal sequences in mammalian cells in culture and in animals. Embryonic stem cells containing recombinase nucleic acid constructs that were expressed in the male germline simplify current protocols for producing mice bearing homologously recombined alleles that have been secondarily rearranged by a site-specific recombinase. In different ProCre strains, between 80% and 100% of the progeny that inherited a Cre target nucleic acid construct from males that were also heterozygous for a ProCre nucleic acid construct inherited the Cre-recombined target. ProCre nucleic acid constructs and recombined targets segregated in the first generation. When ES cells prepared from one ProCre line were transfected with vectors containing a loxP-flanked neomycin cassette, G418 resistant, homologously recombined clones, in which the loxP sites remained functional, were readily isolated.