The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 2006
Filed:
Aug. 29, 2003
Anup Sood, Flemington, NJ (US);
Shiv Kumar, Belle Mead, NJ (US);
John Nelson, Hillsborough, NJ (US);
Carl Fuller, Berkeley Heights, NJ (US);
Anuradha Sekher, Monmouth Junction, NJ (US);
Anup Sood, Flemington, NJ (US);
Shiv Kumar, Belle Mead, NJ (US);
John Nelson, Hillsborough, NJ (US);
Carl Fuller, Berkeley Heights, NJ (US);
Anuradha Sekher, Monmouth Junction, NJ (US);
GE Healthcare Bio-Sciences Corp., Piscataway, NJ (US);
Abstract
The present invention relates generally to the use of terminal-phosphate-labeled nucleotides having three or more phosphates as substrates for nucleic acid polymerases and their use in DNA amplification. The labels employed are chemiluminescent, fluorescent, electrochemical and chromogenic moieties as well as mass tags and include those that are directly detectable, detectable after enzyme activation or feed into other processes to generate a different signal. The signal generated from the attached dyes may also be used to quantify the amount of amplification. Further provided are stabilizers that enhance the stability of terminal-phosphate labeled nucleoside polyphosphates in aqueous solutions and are useful for reducing non-enzymatic hydrolysis of these nucleotides, hence decrease background.