The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 17, 2006
Filed:
Jul. 03, 2001
László Homolya, Budapest, HU;
Balázs Sarkadi, Ágnes st., HU;
Raymond Evers, East Brunswick, NJ (US);
László Homolya, Budapest, HU;
Balázs Sarkadi, Ágnes st., HU;
Raymond Evers, East Brunswick, NJ (US);
Solvo Biotechnology, Szeged, HU;
Abstract
The invention relates to a simple quantitative fluorescent assay method for determining the activity of transport proteins, more specifically multi-drug resistance associated proteins (MRPs). The method of the invention is performed on a well sealed culture of polarized cells expressing a transport protein of interest grown to confluency on a permeable support, said confluent cell culture forming well separated apical and basolateral compartments. The cells are contacted with a cell permeable non-fluorescent derivative of a fluorescent compound (e.g. calcein AM) in one compartment and, after a certain period of incubation, the fluorescence intensity detected in a sample taken from the opposite compartment is indicative of the activity of the transport protein of interest expressed in the cells. The invention also concerns methods for the qualitative and quantitative determination of inhibitors and activators of transport proteins of interest and methods for assessing basolateral and/or apical localization of plasma membrane bound transport proteins of interest in polarized cells.