The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 26, 2006
Filed:
May. 23, 2000
Helena Domingues, Heidelberg, DE;
Hartmut Oschkinat, Berlin, DE;
Luis Serrano, Heidelberg, DE;
Joerg Peters, Elberfeld, DE;
Helena Domingues, Heidelberg, DE;
Hartmut Oschkinat, Berlin, DE;
Luis Serrano, Heidelberg, DE;
Joerg Peters, Elberfeld, DE;
European Molecular Biology Laboratory, Heidelberg, DE;
Bayer AG, Leverkusen, DE;
Abstract
The invention relates to methods for the stabilisation of cytokines and to cytokines generated by such methods. The methods of the invention involve mutating the amino acid sequence of a cytokine so as to remove solvent-exposed hydrophobic residues, and/or mutating the amino acid sequence of the cytokine so as to stabilise one or more secondary structure elements in the molecule. These steps have the effect of destabilsing intermediates that are formed during the folding process, relative to the stability of the cytokine in its naturally folded state, so increasing the yield of the cytokine as produced in vitro.