The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 26, 2006
Filed:
Jun. 10, 2004
Akio Ozaki, Machida, JP;
Hideo Mori, Machida, JP;
Takeshi Shibasaki, Machida, JP;
Katsuhiko Ando, Machida, JP;
Keiko Ochiai, Ebina, JP;
Shigeru Chiba, Kawasaki, JP;
Yoichi Uosaki, Machida, JP;
Akio Ozaki, Machida, JP;
Hideo Mori, Machida, JP;
Takeshi Shibasaki, Machida, JP;
Katsuhiko Ando, Machida, JP;
Keiko Ochiai, Ebina, JP;
Shigeru Chiba, Kawasaki, JP;
Yoichi Uosaki, Machida, JP;
Kyowa Hakko Kogyo Co., Ltd., Tokyo, JP;
Abstract
An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.