The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 2006
Filed:
May. 06, 2002
Steven C. Pruitt, Williamsville, NY (US);
David G. Hangauer, East Amherst, NY (US);
Carleton C. Stewart, Orchard Park, NY (US);
Lawrence Mark Mielnicki, Buffalo, NY (US);
Steven C. Pruitt, Williamsville, NY (US);
David G. Hangauer, East Amherst, NY (US);
Carleton C. Stewart, Orchard Park, NY (US);
Lawrence Mark Mielnicki, Buffalo, NY (US);
The Research Foundation of State University of New York, Amherst, NY (US);
Abstract
The present invention provides a method for screening of a large number of compounds for their ability to modulate the expression of genes. The method uses gene trap technology and comprises the steps of transfecting a population of cells with a gene-trap vector, sorting cells according to their level of fluorescence, distributing sorted cells into pools and expanding the pools to obtain a sufficient number of cells representing each trapped gene to permit distinction of the effect of a test compound over controls, exposing the cells to the test compounds and identifying compounds which alter the fluorescence distribution pattern of cells using FACS analysis.