The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 18, 2006
Filed:
Dec. 12, 2003
Jonathan F. Smith, Cary, NC (US);
Kurt Kamrud, Apex, NC (US);
Sergey Dryga, Chapel Hill, NC (US);
Harold Alterson, Cary, NC (US);
Jon Rayner, Apex, NC (US);
Kim Butler, Cary, NC (US);
Maureen F. Maughan, Durham, NC (US);
Jonathan F. Smith, Cary, NC (US);
Kurt Kamrud, Apex, NC (US);
Sergey Dryga, Chapel Hill, NC (US);
Harold Alterson, Cary, NC (US);
Jon Rayner, Apex, NC (US);
Kim Butler, Cary, NC (US);
Maureen F. Maughan, Durham, NC (US);
AlphaVax, Inc., Research Triangle Park, NC (US);
Abstract
Provided herein are methods for producing alphavirus replicon particles in high yield; replicon RNAs are electroporated into permissive cells, where the cells are at a relatively high density, together with at least one helper nucleic acid providing the necessary functions for packaging. After a growth period in appropriate medium, alphavirus replicon particles are harvested from the surfaces of the cells in which they were produced using a salt wash in which the salt concentration is from about 0.2 to about 5 M sodium chloride, calcium chloride, magnesium chloride, potassium chloride, ammonium acetate, ammonium bicarbonate, among others. After dilution, if necessary, the particles can be purified by a suitable chromatographic technique.