The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 23, 2006
Filed:
Nov. 06, 2001
H. Holden Thorp, Chapel Hill, NC (US);
Dean H. Johnston, Columbus, OH (US);
Mary E. Napier, Carrboro, NC (US);
Carson R. Loomis, Durham, NC (US);
Mark F. Sistare, Chapel Hill, NC (US);
Jinheung Kim, Kyungnam, KR;
H. Holden Thorp, Chapel Hill, NC (US);
Dean H. Johnston, Columbus, OH (US);
Mary E. Napier, Carrboro, NC (US);
Carson R. Loomis, Durham, NC (US);
Mark F. Sistare, Chapel Hill, NC (US);
Jinheung Kim, Kyungnam, KR;
The University of North Carolina at Chapel Hill, Chapel Hill, NC (US);
Abstract
A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.