The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 07, 2006
Filed:
May. 03, 2001
Yuping Ambuel, Madison, WI (US);
Thomas R. Van Oosbree, Madison, WI (US);
Mark R. Mccormick, Madison, WI (US);
Robert C. Mierendorf, Madison, WI (US);
Yuping Ambuel, Madison, WI (US);
Thomas R. Van Oosbree, Madison, WI (US);
Mark R. McCormick, Madison, WI (US);
Robert C. Mierendorf, Madison, WI (US);
EMD Biosciences, Inc., San Diego, CA (US);
Abstract
There is now a market for kits containing the necessary components for performing protein synthesis reactions using template DNA of the experimenter. One category of existing system for performing protein synthesis reactions is based on an S-30 extract from the bacteria. It is disclosed here that a simple fractionation process can dramatically improve the performance of an S-30 prokaryotic protein synthesis reaction mixture. In one embodiment, the fractionation is a simple freezing and thawing of an S-30 extract combined with a supplemental mix, followed by centrifugation. The resulting fractionated S-30 reaction mixture yields more full-length target protein and less non-full length or non-target protein than possible using prior art S-30 systems.