The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 21, 2006
Filed:
Aug. 27, 2004
Solomon H. Snyder, Baltimore, MD (US);
Samie R. Jaffrey, New York, NY (US);
Christopher D. Ferris, Franklin, TN (US);
Hediye Erdjument-bromage, New York, NY (US);
Paul Tempst, New York, NY (US);
Solomon H. Snyder, Baltimore, MD (US);
Samie R. Jaffrey, New York, NY (US);
Christopher D. Ferris, Franklin, TN (US);
Hediye Erdjument-Bromage, New York, NY (US);
Paul Tempst, New York, NY (US);
The Johns Hopkins University, Baltimore, MD (US);
Memorial Sloan-Kettering Cancer Center, New York, NY (US);
Abstract
Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. A method to detect proteins which contain nitrosothiols involves several steps. Nitrosylated cysteines are converted to tagged cysteines. Tagged proteins can then be detected, for example, by immunoblotting and/or can be purified by affinity chromatography. The method is applicable to the detection of S-nitrosylated proteins in cell lysates following in vitro S-nitrosylation, as well as to the detection of endogenous S-nitrosothiols in selected protein substrates.