The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 14, 2006

Filed:

Jun. 21, 2000
Applicants:

Mark Maffitt, Madison, WI (US);

Andrew L. Niles, Madison, WI (US);

Mary Haak-frendscho, Newark, CA (US);

Inventors:

Mark Maffitt, Madison, WI (US);

Andrew L. Niles, Madison, WI (US);

Mary Haak-Frendscho, Newark, CA (US);

Assignee:

Promega Corporation, Madison, WI (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/64 (2006.01); C12N 5/00 (2006.01); C12N 15/00 (2006.01); C12N 1/14 (2006.01); C12P 21/06 (2006.01);
U.S. Cl.
CPC ...
Abstract

Disclosed is a method of expressing enzymatically-active, recombinant proteolytic tryptase in a eukaryotic host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed hosts, and genetically-engineered eukaryotic host cells containing the expression constructs and which express enzymatically-active proteolytic tryptases. Uses for the proteolytic tryptases so produced are also disclosed. Also disclosed is a method of making active site mutants of proteolytic tryptases in a eukaryotic host cell, expression constructs which drive the production of the mutants in transformed eukaryotic host cells, and genetically-engineered eukaryotic host cells containing the expression constructs and which express the active-site mutated form of proteolytic tryptases.


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