The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 07, 2006

Filed:

Oct. 04, 2000
Applicants:

Seiko Hirano, Kawasaki, JP;

Eiichiro Kimura, Kawasaki, JP;

Tsuyoshi Osumi, Kawasaki, JP;

Kazuhiko Matsui, Kawasaki, JP;

Yoshio Kawahara, Kawasaki, JP;

Gen Nonaka, Kawasaki, JP;

Yumi Matsuzaki, Kawasaki, JP;

Naoki Akiyoshi, Kawasaki, JP;

Kanae Nakamura, Ho Chiminh, VN;

Osamu Kurahashi, Kawasaki, JP;

Tsuyoshi Nakamatsu, Tokyo, JP;

Shinichi Sugimoto, Kawasaki, JP;

Inventors:

Seiko Hirano, Kawasaki, JP;

Eiichiro Kimura, Kawasaki, JP;

Tsuyoshi Osumi, Kawasaki, JP;

Kazuhiko Matsui, Kawasaki, JP;

Yoshio Kawahara, Kawasaki, JP;

Gen Nonaka, Kawasaki, JP;

Yumi Matsuzaki, Kawasaki, JP;

Naoki Akiyoshi, Kawasaki, JP;

Kanae Nakamura, Ho Chiminh, VN;

Osamu Kurahashi, Kawasaki, JP;

Tsuyoshi Nakamatsu, Tokyo, JP;

Shinichi Sugimoto, Kawasaki, JP;

Assignee:

Ajinomoto Co., Inc., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12P 21/06 (2006.01);
U.S. Cl.
CPC ...
Abstract

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from, preferably an enzyme that functions at a higher temperature compared with that of. PCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of


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