The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 07, 2006
Filed:
Jul. 21, 2003
Paola Capodieci, New York, NY (US);
Jon Edelson, Scarsdale, NY (US);
Paola Capodieci, New York, NY (US);
Jon Edelson, Scarsdale, NY (US);
Aureon Laboratories, Inc., Yonkers, NY (US);
Abstract
This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by 'mRNA liberation in fixed-treated tissue or 'MLIFTT''. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence and the physical treatments overcome the interference created by the fixation-induced chemical bonds. The methods of the present invention can be utilized to identify a plurality of mRNA transcripts in a microarray format.