The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 29, 2005
Filed:
Apr. 30, 2002
John C. Olsen, Chapel Hill, NC (US);
Kyriacos Andreou Mitrophanous, Oxford, GB;
Jonathan Rohll, San Diego, CA (US);
Alan John Kingsman, Oxford, GB;
Fiona Margaret Ellard, Berkshire, GB;
John C. Olsen, Chapel Hill, NC (US);
Kyriacos Andreou Mitrophanous, Oxford, GB;
Jonathan Rohll, San Diego, CA (US);
Alan John Kingsman, Oxford, GB;
Fiona Margaret Ellard, Berkshire, GB;
Oxford Biomedica (UK) Limited, Oxford, GB;
The University of North Carolina at Chapel Hill, Chapel Hill, NC (US);
Abstract
A method for producing viral vectors is described using packaging and producer cell lines is described. The producer cell comprises: (i) a first nucleotide sequence (NS) encoding a toxic viral envelope protein operably linked to a promoter; wherein the promoter is operably linked to at least one copy of a TRE; (ii) a second NS wherein the second NS comprises a sequence encoding a tetracycline modulator; (iii) a third NS encoding a retrovirus nucleocapsid protein; and (iv) a fourth NS comprising a retroviral sequence capable of being encapsidated in the nucleocapsid protein such that the retroviral vector particle titre obtainable from the producer cell is regulatable by tetracycline and an initial stimulus with sodium butyrate or functional analogues thereof.