The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 30, 2005
Filed:
Aug. 07, 2000
Applicants:
Günter Stempfer, Kramsach, AT;
Jörg Windisch, Kramsach, AT;
Franz Knauseder, Kirchbichl, AT;
Inventors:
Assignee:
Sandoz AG, Basel, CH;
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N009/48 ; C12N001/20 ; C12P021/06 ; C12Q001/70 ; C07K001/00 ;
U.S. Cl.
CPC ...
Abstract
The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogenous N-terminus in a bacteria host cell. A fusion comprising a peptide with the autoproteolytic activity of an autoprotease Nof a pestivirus and the heterologous polypeptide is initially expressed in the form of cytoplasmic inclusion bodies in the host cell, the inclusion bodies are isolated and subsequently treated so that the desired heterologous polypeptide is cleaved autoproteolytically by the Nactivity of the fusion protein.