The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 2005
Filed:
May. 03, 2000
Timo Korpela, Turku, FI;
Sheila Macintyre-ayane, Reading, GB;
Anton Zavialov, Moscow, RU;
Natalia Battchikova, Turku, FI;
Lada Petrovskaya, Moscow, RU;
Vladimir Zav'yalov, Moscow, RU;
Galina Petrovna Korobko, Moscow, RU;
Timo Korpela, Turku, FI;
Sheila Macintyre-Ayane, Reading, GB;
Anton Zavialov, Moscow, RU;
Natalia Battchikova, Turku, FI;
Lada Petrovskaya, Moscow, RU;
Vladimir Zav'yalov, Moscow, RU;
Galina Petrovna Korobko, Moscow, RU;
Biotecnol S.A., Oeiras, PT;
Abstract
The present invention provides bacterial strains for secretion of soluble biologically active recombinant heterologous proteins into periplasm or on a surface/into a cultivation medium of bacteria. The invention exploits the secretion system of Gram-negative bacteria including periplasmic chaperones and usher/secretin proteins. For accomplishing the aim, the bacterial strains simultaneously express the fusion protein (signal peptide)-(mature hecterologous protein)-(subunit of a bacterial surface structure, Caf1), periplasmic chaperone specific for the subunit, and outer membrane usher/secretin protein specific for the subunit. Secretion of fusion proteins: (signal peptide of Caf1)-(mature human IL-1β)-(mature Caf1), (signal peptide of Caf1)-(mature human GM-CSF)-(mature Caf1), and (signal peptide of Caf1)-(mature human IL-1ra)-(mature Caf1) that were expressed insimultaneously with the periplasmic chaperone Caf1M and the usher/secretin protein Caf1A are examples of the use of the invention.