The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 26, 2005
Filed:
Jul. 23, 2001
Rainer Mueller, Penzberg, DE;
Johann-peter Thalhofer, Weilheim, DE;
Frank Geipel, Penzberg, DE;
Werner Hoelke, Penzberg, DE;
Stephan Glaser, Seeshaupt, DE;
Hellmut Eckstein, Weilheim, DE;
Thomas Kirschbaum, Munich, DE;
Bettina Bommarius, Atlanta, GA (US);
Rainer Mueller, Penzberg, DE;
Johann-Peter Thalhofer, Weilheim, DE;
Frank Geipel, Penzberg, DE;
Werner Hoelke, Penzberg, DE;
Stephan Glaser, Seeshaupt, DE;
Hellmut Eckstein, Weilheim, DE;
Thomas Kirschbaum, Munich, DE;
Bettina Bommarius, Atlanta, GA (US);
Roche Diagnostics Operations, Inc., Indianapolis, IN (US);
Abstract
This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.