The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 15, 2005
Filed:
Feb. 09, 2001
Lance A. Liotta, Potomac, MD (US);
Michael Emmert-buck, Silver Spring, MD (US);
David B. Krizman, Gaithersburg, MD (US);
Rodrigo Chuaqui, Las Condes, CL;
W. Marston Linehan, North Bethesda, MD (US);
Jeffry M. Trent, Rockville, MD (US);
Robert F. Bonner, Washington, DC (US);
Seth R. Goldstein, Bethesda, MD (US);
Paul D. Smith, Annapolis, MD (US);
John I. Peterson, Falls Church, VA (US);
Lance A. Liotta, Potomac, MD (US);
Michael Emmert-Buck, Silver Spring, MD (US);
David B. Krizman, Gaithersburg, MD (US);
Rodrigo Chuaqui, Las Condes, CL;
W. Marston Linehan, North Bethesda, MD (US);
Jeffry M. Trent, Rockville, MD (US);
Robert F. Bonner, Washington, DC (US);
Seth R. Goldstein, Bethesda, MD (US);
Paul D. Smith, Annapolis, MD (US);
John I. Peterson, Falls Church, VA (US);
Abstract
A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated. During separation, the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample, the zone of cells of interest may then be molecularly analyzed.