The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2005
Filed:
Apr. 05, 2000
Aaron P. White, Victoria, CA;
James L. Doran, Brentwood Bay, CA;
S. Karen Collinson, Brentwood Bay, CA;
William W. Kay, Victoria, CA;
Aaron P. White, Victoria, CA;
James L. Doran, Brentwood Bay, CA;
S. Karen Collinson, Brentwood Bay, CA;
William W. Kay, Victoria, CA;
Abstract
The invention provides a system for creating recombinant agfA fimbrin genes and performing chromosomal gene replacements within, creatingstrains which carry the recombinant agfA genes at the native position in the chromosome. One embodiment of the invention is exemplified by the expression of a model epitope (PT3) obtained from the GP63 protein of, by formation of recombinant agfA genes encoding PT3 fusion proteins recombined at 10 different sites throughout the agfA gene. These fusions are shown to be expressed in the thin aggregative fimbriae on the surface of bacterial cell. The agfA fimbrin of(CsgA for) provides a flexible and stable vehicle for the expression of foreign epitopes in enterobacteriaceae and the subsequent thin aggregative fimbriae (curli) expression product provide an ideal organelle for presentation of the foreign epitopes at the cell surface.