The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 01, 2005
Filed:
Oct. 24, 2001
Grigori N. Enikolopov, Cold Spring Harbor, NY (US);
Natalia I. Peunova, Cold Spring Harbor, NY (US);
Boris A. Kuzin, Moscow, RU;
Hollis Cline, Cold Spring Harbor, NY (US);
Tatyana Michurina, Moscow, RU;
Grigori N. Enikolopov, Cold Spring Harbor, NY (US);
Natalia I. Peunova, Cold Spring Harbor, NY (US);
Boris A. Kuzin, Moscow, RU;
Hollis Cline, Cold Spring Harbor, NY (US);
Tatyana Michurina, Moscow, RU;
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (US);
Abstract
The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation in hematopoietic tissue, wherein the hematopoietic tissue is contacted with at least one inhibitor of NO, such as one or more inhibitors of nitric oxide synthase (NOS), thereby producing hematopoietic tissue having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor (one or more) of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g., blood, skin, bone and digestive epithelium), or precursor cells of the selected tissue, with an inhibitor (one or more) of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue.