The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 19, 2004
Filed:
Oct. 03, 2001
Applicant:
Inventors:
Karl Bayer, Vienna, AT;
Reingard Grabherr, Pressbaum, AT;
Erik Nilsson, Linkoeping, SE;
Gerald Striedner, Vienna, AT;
Assignee:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 2/100 ; C07H 2/104 ; C12M 1/500 ;
U.S. Cl.
CPC ...
C12P 2/100 ; C07H 2/104 ; C12M 1/500 ;
Abstract
In an expression vector having a ColE1 replication system, the homology of the RNAI and RNAII of the ColE1 origin of replication to uncharged tRNAs is modified mutations in the coding region of the RNAI gene and corresponding mutations in the RNAII gene. The mutation results in one or more base exchanges in loop 1 and/or loop 2 and/or loop 3 of RNAI and RNAII. In methods using this vector for producing recombinant proteins, plasmid copy number is stably maintained. In methods for plasmid production, high plasmid copy numbers can be obtained.