The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 01, 2004

Filed:

Oct. 25, 2001
Applicant:
Inventors:

John P. Marino, Silver Spring, MD (US);

James T. Stivers, Baltimore, MD (US);

Karen Lacourciere, Silver Spring, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; G01N 3/353 ; G01N 3/3566 ; C07K 1/400 ; C07H 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; G01N 3/353 ; G01N 3/3566 ; C07K 1/400 ; C07H 2/102 ; C07H 2/104 ;
Abstract

The present invention is a method of identifying ligands that interact will cellular processes involved in the life-cycle of HIV. In particular, an oligonucleotide, corresponding to specific RNA sequence within an infected cell, is modified by the substitution of 2-aminopurine. As a result, interactions between the oligonucleotide and the ligand can be measured via fluorescence. This technique can be use to find inhibitors of binding between rev and its response element (RRE), dimerization initiation sequences, and topoisomerases and DNA.


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