The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 27, 2004
Filed:
Dec. 29, 2000
Applicant:
Inventors:
Brian D. Kelley, Medford, MA (US);
Suresh Vunnum, North Andover, MA (US);
Johanna Dalborg, Stockholm, SE;
Anna Petersson, Enskede, SE;
Assignee:
Genetics Institute, LLC, Cambridge, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 1/4755 ; C07K 1/18 ; C07K 1/22 ;
U.S. Cl.
CPC ...
C07K 1/4755 ; C07K 1/18 ; C07K 1/22 ;
Abstract
Methods are provided for purification of Factor VIII polypeptides by immunoaffinity chromatography and ion exchange chromatography, in which the eluate from the immunoaffinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The methods result in improved purification without significant yield loss.