The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 30, 2003

Filed:

Jun. 21, 2001
Applicant:
Inventors:

Ronald H. Chiarello, Castro Valley, CA (US);

Sarah Himmerich, San Francisco, CA (US);

Assignee:

BASF AG, Ludwigshafen, DE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/04 ; C12N 5/00 ; C12N 5/02 ; C12N 5/04 ; C12N 5/10 ;
U.S. Cl.
CPC ...
C12N 1/04 ; C12N 5/00 ; C12N 5/02 ; C12N 5/04 ; C12N 5/10 ;
Abstract

A serum-free insect cell culture medium is provided which provides improvements in the maximum insect cell density and replication of insect viruses within these cells, at a significantly lower cost than commercially-available media. A method is provided for preparing a lipid emulsion for use in insect culture media for large scale culture, and which produces a more stable emulsion with a longer shelf life. The method is preferably carried out by combining in an organic solvent a surfactant and a mixture of lipids to make a lipid phase, heating the lipid phase to a temperature of 40 to 70 degrees Celsius to form an anhydrous lipid phase and adding an aqueous phase thereto to provide a stable lipid micro-emulsion.


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