The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 22, 2002
Filed:
Jun. 14, 2001
Applicant:
Inventors:
Michael Egholm, Woodbridge, CT (US);
Caifu Chen, Palo Alto, CA (US);
Assignee:
PE Corporation, Foster City, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 2/100 ; C07H 2/102 ; C07H 2/104 ; C12Q 1/68 ;
U.S. Cl.
CPC ...
C07H 2/100 ; C07H 2/102 ; C07H 2/104 ; C12Q 1/68 ;
Abstract
The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3′ hydroxyl or 5′ hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.