The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 24, 2002
Filed:
Oct. 26, 2000
Applicant:
Inventors:
Douglas T. Gjerde, Saratoga, CA (US);
Paul D. Taylor, Gilroy, CA (US);
Robert M. Haefele, Campbell, CA (US);
Assignee:
Transgenomic, Inc., San Jose, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 2/104 ; C07H 2/102 ; C12Q 1/68 ; C12Q 1/934 ; B01D 1/501 ;
U.S. Cl.
CPC ...
C07H 2/104 ; C07H 2/102 ; C12Q 1/68 ; C12Q 1/934 ; B01D 1/501 ;
Abstract
The present invention is directed to improved methods for detection of mutations in DNA using Denaturing Matched Ion Polynucleotide Chromatography (DMIPC). The invention includes the following aspects: analysis of PCR amplification products to identify factors that affect PCR replication fidelity; design of PCR primers; selection of an optimal temperature for performing DMIPC; selection of the mobile phase composition for gradient elution; methods for column preparation and maintenance; and methods for preparing polynucleotide samples prior to chromatographic analysis.