The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 2002
Filed:
Oct. 28, 1999
Applicant:
Inventors:
Shuang-yong Xu, Lexington, MA (US);
Jian-ping Xiao, Wenham, MA (US);
Assignee:
New England Biolabs, Inc., Beverly, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 ; C12N 1/555 ;
U.S. Cl.
CPC ...
C12N 9/22 ; C12N 1/555 ;
Abstract
The present invention relates to recombinant DNA which encodes the NheI restriction endonuclease as well as NheI methyltransferase, expression of NheI restriction endonuclease from cells containing the recombinant DNA. An internal NdeI site in the nheIR gene was eliminated by a silent mutation. A new NdeI site was engineered at the start codon of nheIR gene. An NdeI-BamHI fragment containing nheIR gene was cloned into a T7 expression vector pAII17 and expressed in a premodified host ER2566 [pACYC-NheIM, pAII17-NheIR2]. The recombinant clone produces approximately 10 million units of NheI per gram of wet cells.