The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2002
Filed:
Oct. 29, 1999
Karl-Heinz Drexhage, D-57076 Siegen, DE;
Jürgen Wolfrum, D-37127 Rosdorf, DE;
Other;
Abstract
In order to carry out optical detection and identification of individual tumor markers in undiluted blood plasma, said tumor markers are marked with fluorescent dyes which are characterized by specific fluorescent lifetimes ranging from 0.5 to 6. The blood plasma is exposed to pulsed diode laser radiation emitted in the 630 to 670 nm wavelength range. The emission wavelengths of the dyes are selected in such a way that they are 10 to 60 nm longer than the wavelength of the diode laser. Blood plasma decay curves are detected using time-correlated single photon counts. Said curves are described by a bi-exponential model wherein it is assumed that a fixed fluorescent lifetime for blood plasma emission is 300 ps and that the marked tumor markers have a variable fluorescent lifetime. The relative amount of fluorescent photons in the marked tumors is determined in relation to the overall number of photons detected. If this value exceeds 0.3, a tumor marker is present in the observed volume. The tumor markers can also be identified by the lifetimes which are determined through bi-exponential evaluation. In this manner, individual tumor markers can be detected and identified in undiluted blood plasma.