The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 23, 2002
Filed:
Dec. 16, 1999
Joseph Atabekov, Moscow 117311, RU;
Timo Korpela, Turku, FI;
Yurii Dorokhov, Moscow 117321, RU;
Peter Ivanov, Moscow 117312, RU;
Maxim Skulachev, Moscow 117234, RU;
Nina Rodionova, Moscow 117607, RU;
Olga Karpova, Moscow 117335, RU;
Other;
Abstract
The present invention is drawn to a recombinant nucleic acid having (a) a transcriptional promoter; (b) a first plant-expressible structural gene linked to the transcriptional promoter, (c) a cDNA sequence element having an internal ribosome entry site of tobamovirus moving protein (MP) gene (IRES ) which is located 3′ to the first plant-expressible structural gene, and (d) a second plant-expressible gene located 3′ to the (IRES ) such that the second gene is placed under the translational control of (IRES ); wherein the first plant-expressible gene, (IRES ) and the second plant-expressible gene are transcribed under the action of the transcriptional promoter to give a primary transcript, and the first plant expressible gene of the primary transcript is able to translate by ribosome scanning mechanism and the second plant expressible gene of the primary transcript is capable of translation under the action of (IRES ). The present invention is further drawn to a method of simultaneously expressing desired genes in vitro and in planta by using crTMV RNA sequences upstream or the MP gene, i.e. IRES .