The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 23, 2002
Filed:
Mar. 16, 1998
Pamela Hawley-Nelson, Silver Spring, MD (US);
Jianqing Lan, Germantown, MD (US);
PoJen Shih, Columbia, MD (US);
Joel A. Jessee, Mt. Airy, MD (US);
Kevin P. Schifferli, Germantown, MD (US);
Gulilat Gebeyehu, Silver Spring, MD (US);
Valentina C. Ciccarone, Gaithersburg, MD (US);
Krista L. Evans, Germantown, MD (US);
Life Technologies, Inc., Rockville, MD (US);
Abstract
The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.