The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 15, 2002
Filed:
May. 07, 1999
Applicant:
Inventors:
Ricaredo Matanguihan, Walnut Creek, CA (US);
Eva Sajan, Oakland, CA (US);
Konstantin Konstantinov, Walnut Creek, CA (US);
Michael Zachariou, Walnut Creek, CA (US);
Charles Olson, Moraga, CA (US);
Assignee:
Bayer Corporation, Berkeley, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 ; C12N 5/02 ; C12N 5/06 ; C12N 5/08 ;
U.S. Cl.
CPC ...
C12N 5/00 ; C12N 5/02 ; C12N 5/06 ; C12N 5/08 ;
Abstract
A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.