The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 08, 2002
Filed:
Mar. 30, 2000
Dharam Chand Jain, Lucknow, IN;
Neerja Pant, Lucknow, IN;
Madan Mohan Gupta, Lucknow, IN;
Rajendra Singh Bhakuni, Lucknow, IN;
Ram Kishor Verma, Lucknow, IN;
Sudeep Tandon, Lucknow, IN;
Shiv Kumar Gupta, Lucknow, IN;
Amit Tewari, Lucknow, IN;
Atul Prakash Kahol, Lucknow, IN;
Sushil Kumar, Lucknow, IN;
Council of Scientific and Industrial Research, New Dehli, IN;
Abstract
The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.