The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 25, 2001
Filed:
Jun. 06, 1995
Applicant:
Inventors:
Dennis G. Kleid, San Mateo, CA (US);
Daniel G. Yansura, San Francisco, CA (US);
Herbert L. Heyneker, Burlingame, CA (US);
Giuseppe F. Miozzari, San Carlos, CA (US);
Assignee:
Genentech, Inc., South San Francisco, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/563 ; C12N 1/571 ;
U.S. Cl.
CPC ...
C12N 1/563 ; C12N 1/571 ;
Abstract
Methods for the controllable expression in high yields of heterologous proteins under the control of a modified tryptophan promoter-operator system are disclosed. The system employed involves sequential resort to tryptophan repression and derepression, as well as deletion of an attenuator function native to the tryptophan operon. Means are also disclosed for effecting deletions at any given point within a gene fragment and for the ready identification of transformant bacteria via triple ligation procedures.