The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 18, 2001
Filed:
Nov. 28, 1995
Scott M. Glaser, San Diego, CA (US);
William D. Huse, Del Mar, CA (US);
William P. MacConnell, Cardiff, CA (US);
Ixsys, Inc., San Diego, CA (US);
Abstract
A high throughput device is provided for simultaneously isolating periplasmic fractions from multiple samples of host cells. The device can be formatted to operate in a series. A method for simultaneously isolating multiple periplasmic fractions is also provided. The method includes (a) providing a high throughput device for isolating periplasmic fractions from multiple samples of host cells having an outer membrane via attaching a removable sample chamber having a membrane chamber wall to a vacuum chamber and applying a vacuum thereto (b) removing media with the vacuum and (c) removing the outer membrane of the host cells (d) attaching a collection chamber to the vacuum chamber and applying a vacuum thereto in order to carry out a collection of the periplasmic fractions that pass through the membrane of the chamber wall from the host cells. A workstation is also provided which includes one or more high throughput devices for simultaneously isolating periplasmic space fractions from a plurality of samples of host cells, and a device for simultaneously culturing a plurality of samples. preferably the membrane of the chamber is a neutral nylon membrane having an average pore diameter of,to,micron.