The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 04, 2001

Filed:

Apr. 06, 1999
Applicant:
Inventors:

Wolf-Georg Forssmann, Hannover, DE;

Manfred Raida, Hannover, DE;

Bernhard Brenner, Hannover, DE;

Volker Nier, Hannover, DE;

Assignee:

Pharis Biotec GmbH, Hannover, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/00 ; C12Q 1/37 ;
U.S. Cl.
CPC ...
C12Q 1/00 ; C12Q 1/37 ;
Abstract

The present invention relates to a quick method for the qualitative and quantitative medical-diagnostic analysis on the protein level of the substitution of single amino acids with pathogenic and non-pathogenic effects on the organism. The medical-diagnostic analysis is performed by a combination of enzymatic or chemical cleavage of the isolated peptide, chromatographical separation of the fragments and analysis by mass spectrometry, both direct LC/MS and indirect MALDI-MS, and analysis by capillary electrophoresis. By comparing protein samples from healthy humans with those of ill humans, the method described is suitable for establishing new, as yet unknown mutations and quantifying the expression and incorporation of wild type to mutant.


Find Patent Forward Citations

Loading…