The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 25, 2001
Filed:
Mar. 09, 2000
Michael T. Boyce-Jacino, Finksburg, MD (US);
Yu-Hui Rogers, Damascus, MD (US);
Philip Goelet, Reisterstown, MD (US);
Orchid Biosciences, Inc., Princeton, NJ (US);
Abstract
The present invention provides a method for determining the nucleotide sequence of a nucleic acid molecule using primer oligonucleotides arrayed on a solid support and doing primer extension. By providing sequence information from both oligonucleotide primers in which the 3′ terminal nucleotide of the oligonucleotide primer is hybridized to the nucleic acid molecule and oligonucleotide primers in which the 3′ terminal nucleotide is not hybridized to the nucleic acid molecule the invention is advantageous in that less primers are needed to fully sequence a nucleic acid molecule. Where the 3′ terminal nucleotide of an oligonucleotide in the array does not hybridize to the nucleic acid molecule, the present invention provides for either not permitting oligonucleotides hybridized to nucleic acid molecules to be extended by polymerase-mediated incorporation of a single chain terminator nucleotide residue onto the 3′ terminus of said hybridized oligonucleotide, or permitting the removal of any non-hybridized nucleotide residues from the 3′ terminus of said hybridized oligonucloetides, so as to form a truncated primer oligonucleotide whose 3′ terminus is hybridized to said nucleic acid molecule. The invention also provides kits that can be used to implement the methods of the invention.