The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 11, 2001
Filed:
Oct. 28, 1998
Applicant:
Inventors:
John P. Nolan, Santa Fe, NM (US);
P. Scott White, Los Alamos, NM (US);
Hong Cai, Los Alamos, NM (US);
Assignee:
The Regents of the University of California, Los Alamos, NM (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12P 1/500 ; C07H 2/102 ; C07H 2/104 ; C12N 1/500 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12P 1/500 ; C07H 2/102 ; C07H 2/104 ; C12N 1/500 ;
Abstract
DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.