The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 15, 2001
Filed:
Jan. 19, 1996
Applicant:
Inventors:
Guy Miller, Mountain View, CA (US);
Margaret A. Hirst, Redwood City, CA (US);
Assignee:
Galileo Laboratories, Inc., Santa Clara, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/00 ; C12Q 1/26 ; G01N 3/3566 ;
U.S. Cl.
CPC ...
C12Q 1/00 ; C12Q 1/26 ; G01N 3/3566 ;
Abstract
Methods and compositions are provided for screening compounds for anti-stress activity. A rat hepatoma-derived cell line is employed, which is particularly responsive to mitochondrial function inhibiting agents, demonstrated by the extracellular release of lactate dehydrogenase and the formation of lactate. Cytoprotectant agents are shown to reverse the lactate dehydrogenase release in the presence of mitochondrial function interfering agents, as a model for the effects of hypoxia on cellular energetic function.