The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 08, 2001

Filed:

May. 16, 1995
Applicant:
Inventors:

Barbara Chapman, Berkeley, CA (US);

Rae Lyn Burke, San Francisco, CA (US);

Mirella Ezban Rasmussen, Copenhagen, DK;

Jan Moller Mikkelson, Gentofte, DK;

Assignee:

Other;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 2/102 ; C12N 1/00 ; C12N 5/10 ; C12N 1/512 ; C12N 1/563 ;
U.S. Cl.
CPC ...
C12P 2/102 ; C12N 1/00 ; C12N 5/10 ; C12N 1/512 ; C12N 1/563 ;
Abstract

Recombinant protein complexes having human Factor VIII:C activity are expressed in a eukaryotic host cell by transforming the host cell with first and second expression cassettes encoding a first polypeptide substantially homologous to human Factor VIII:C A domain and a second polypeptide substantially homologous to human Factor VIII:C C domain, respectively. In the present invention, the first polypeptide may be extended having at its C-terminal a human Factor VIII:C B domain N-terminal peptide, a polypeptide spacer of 3-40 amino acids, and a human Factor VIII:C B domain C-terminal peptide. Expression of the second polypeptide is improved by employing an &agr;,-antitrypsin signal sequence.


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