The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 17, 2001

Filed:

May. 26, 1999
Applicant:
Inventors:

Ferhat Ilhan, Atahesir, DE;

Dieter Kraemer, Mainz, DE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 3/704 ; C12P 3/504 ; C12N 1/114 ; C12N 1/102 ; C12N 1/108 ;
U.S. Cl.
CPC ...
C12P 3/704 ; C12P 3/504 ; C12N 1/114 ; C12N 1/102 ; C12N 1/108 ;
Abstract

Beta-lactam antibiotics are synthesized by reacting an amino-beta-lactam component with a corresponding amino-group-containing acylating side-chain component in the presence of penicillin amidase from,covalently immobilized on support particles. The resulting beta-lactam antibiotic product is solubilized by adding an acid such as sulfuric acid to lower the pH to 1.0 at a temperature in the range of 0° C. to +5° C. The immobilized penicillin amidase is substantially inactivated by the acid. After separating the beta-lactam antibiotic product, the immobilized penicillin amidase is substantially reactivated for reuse in antibiotic synthesis by treatment with a buffer having about a neutral pH. Antibiotics that can be produced include ampicillin, amoxicillin, cephalexin, cefaclor and cefadroxil. Support particles that can be used include particles having a macroporous structure and a particle diameter of 10-1000 &mgr;m, particles having oxirane groups, particles made of a synthetic polymer and inorganic particles such as porous glass particles.


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