Process for producing the enzyme d-amino acid oxidase of rhodotorula gracilis in host cells

Abstract

Process for producing the enzyme D-amino acid oxydase of,in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of,producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of,with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.