The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 06, 2001
Filed:
May. 25, 1999
Alan F. Corin, Sudbury, MA (US);
Andrew A. Pakula, Lexington, MA (US);
Julianne Bryan, Medford, MA (US);
ANADYS Pharmaceuticals, Inc., Waltham, MA (US);
Abstract
The present invention is directed to a method for selecting compounds for use in treating sickle cell disease in a patient. The method comprises the steps of selecting a first set of HbS ligands from a first group of chemical compounds, selecting from the first set of HbS ligands a seconds set that exhibit inhibition of HbS polymerization, and selecting from the second set a third set that display anti-sickling activity of red blood cells when in the presence thereof. The method may include the step of performing a methemoglobin S proteolysis assay measuring a percent protease protection for each compound. The method may additionally include the steps of measuring HbS aggregation, inhibition of protease, deoxy-HbS gelation inhibition activity, O,affinity of HbS, and cytotoxic effect to cell activity. The method may further include assessing red blood cell morphology, and eliminating compounds that contain moieties that bind to heme. The steps of the method may be repeated on analogs of compounds that are selected using one or more steps of the method. The present invention also includes a method of treating sickle cell disease in a patient, which comprises administering to the patient a pharmaceutical formulation including a selected concentration of an HbS ligand and a pharmaceutically acceptable carrier therefore. The HbS ligand, which may be an isoquinolium derivative, is one exhibiting inhibition of HbS polymerization and displaying anti-sickling activity of red blood cells when in the presence thereof.