The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 17, 2000
Filed:
Jan. 21, 1998
Vladimir Georgievich Debabov, Moscow, RU;
Uri Ivanovich Kozlov, Moscow, RU;
Evgenyi Moiseevich Khurges, Moscow, RU;
Vytalyi Arkadievich Lifshits, Moscow, RU;
Nelli Isaakovna Zhdanova, Moscow, RU;
Michail Markovich Gusyatiner, Moscow, RU;
Alexander Konstantinovich Sokolov, Moscow, RU;
Tatiana Alexandrovna Bachina, Moscow, RU;
Andrei Yurevich Christoserdov, Moscow, RU;
Uri Dmitrievich Tsigankov, Moscow, RU;
Nikolai Kazimirovich Yankovsky, Moscow, RU;
Sergi Vladimirovich Mashko, Moscow, RU;
Alla Lvovna Lapidus, Moscow, RU;
Oksana Fedorovna Gavrilova, Moscow, RU;
Oleg Alexandrovich Rodionov, Moscow, RU;
Ajinomoto Co., Inc., Tokyo, JP;
Abstract
The present invention provides a novel microbial strain and a method for effectively producing L-threonine. The novel strain can grow on a medium containing molasses, a much cheaper raw material than sucrose. The exemplary novel strain E. coli BKIIM B-5318 bears the plasmid pPRT614, which has threonine biosynthesis genes (thr A, B and C), the expression of which is regulated by a lambda-phage PR promoter and temperature-sensitive C1 repressor. The present microorganism is prototrophic with regard to isoleucine. The strain E. coli BKIIM B-5318 produces more than 70 g/l of L-threonine when cultured at 38-41.degree. C. for 32 hours in a medium containing molasses.