The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2000
Filed:
Jan. 28, 1999
Applicant:
Inventors:
Thomas J Burke, Madison, WI (US);
Randall E Bolger, Oregon, WI (US);
Francis J Lenoch, Madison, WI (US);
Assignee:
Panvera, Inc., Madison, WI (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C07K / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 530350 ; 530358 ; 536 231 ; 536 243 ;
Abstract
Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.