The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 25, 2000

Filed:

Nov. 23, 1988
Applicant:
Inventors:

Mel N Kronick, Palo Alto, CA (US);

Douglas H Keith, Oakland, CA (US);

Lincoln J McBride, Redwood City, CA (US);

Norman M Whiteley, San Carlos, CA (US);

Michael W Hunkapiller, San Carlos, CA (US);

Assignee:

Applied Biosystems, Inc., Foster City, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435-772 ; 536 231 ; 536 242 ; 536 2431 ; 536 2432 ;
Abstract

Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nucleic acid from free and non-specifically immobilized nucleic acid, releasing specifically immobilized nucleic acid, and detecting the presence of the sequence of interest by means of the label. The labeled sequence may be characterized by sizing, e.g. electrophoresis. The method provides for a sensitive and rapid means for accurate detection of sequences of interest in a wide variety of situations.


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