The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2000

Filed:

Sep. 19, 1997
Applicant:
Inventors:

Jay S Stout, Lincoln, NE (US);

Bruce E Partridge, Lincoln, NE (US);

Dennis B Henriksen, Lincoln, NE (US);

Barton Holmquist, Lincoln, NE (US);

Fred W Wagner, Walton, NE (US);

Assignee:

BioNebraska, Inc., Lincoln, NE (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C07K / ; C07H / ;
U.S. Cl.
CPC ...
435 691 ; 435 681 ; 435 694 ; 435 697 ; 435 698 ; 530308 ; 530324 ; 530345 ; 536 234 ;
Abstract

A method for the production of C-terminal amidated recombinant peptides is provided. The method employs a recombinant protein construct having multiple copies of a target peptide linked by intraconnecting peptides. The intraconnecting peptides permit the multicopy construct to be selectively reacted to produce product peptides having a C-terminal .alpha.-carboxamide. A recombinant gene containing a DNA sequence coding for the recombinant protein construct and an expression cassette, an expression vector and a transformed cell including the recombinant gene are also provided.


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