The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2000

Filed:

Nov. 07, 1997
Applicant:
Inventors:

Sean A McCarthy, Boston, MA (US);

Michael Joseph Kuranda, Acton, MA (US);

Christine Ellen Bulawa, Arlington, MA (US);

Steven Bossone, Lexington, MA (US);

Assignee:

Millennium BioTherapeutics, Inc., Cambridge, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435 699 ; 435 914 ; 4352551 ; 4353201 ; 536 234 ; 536 2374 ; 536 241 ;
Abstract

The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.


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