The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 21, 2000

Filed:

May. 07, 1997
Applicant:
Inventor:

Karl K Johe, Potomac, MD (US);

Assignee:

NeuralStem Biopharmaceuticals, Ltd., College Park, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
435377 ; 435325 ; 435368 ; 435353 ;
Abstract

The present invention reveals in vitro cultures of region-specific, terminally differentiated, mature neurons derived from cultures of mammalian multipotential CNS stem cells and an in vitro procedure by which the differentiated neurons may be generated. The procedure involves the culturing of multipotential CNS stem cells from a specific region in a chemically defined serum-free culture medium containing a growth factor; replacing the medium with growth factor-free medium; harvesting the stem cells by trypsinization; plating the stem cells at a density of between 100,000 to 250,000 cells per square centimeter; and culturing the stem cells in a glutamic acid-free chemically defined serum-free culture medium.


Find Patent Forward Citations

Johe, Karl. (2000). In vitro generation of differentiated neurons from cultures of mammalian (U.S. Patent No. 6040180). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/6040180

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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